Contact form. LinkList ul li ul'. LazyIfy on Scroll by Templateify v1. TickerIfy by Templateify v1. Leading strand. Lagging strand. A primer is a short sequence of complementary base pairing, to get the whole system running. However, the lagging strand runs in the opposite direction, from 3prime to 5prime. This means nucleotides can only be added discontinuously. RNA primers have to attach where they can, which is at different parts along the strand, to be able to initiate strand synthesis.
Generally, Okazaki fragments are short segments of DNA, about nucleotides long. Figure 2: Continuity of the Leading and Lagging Strand. Also, at the beginning of each Okazaki fragment, an RNA primer has to be synthesized on the lagging strand. Thus, this is the main difference between leading and lagging strand.
Importantly, the leading strand grows continuously while the lagging strand grows discontinuously by forming Okazaki fragments. Leading strand requires a single primer for the synthesis while the lagging strand requires a new primer to start each Okazaki fragment. Hence, this is another difference between leading and lagging strand. Moreover, the starting point too contributes to the difference between leading and lagging strand.
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